The microfluidic components market is experiencing significant growth, expected to reach USD 12.17 billion by 2032.
🔹 Top Companies:
Danaher Corporation
Illumina Inc.
PerkinElmer Inc.
Agilent Technologies Inc.
Bio-Rad Laboratories Inc.
Thermo Fisher Scientific Inc.
For detailed insights, view the full report: https://www.credenceresearch.com/report/microfluidic-components-market
#Microfluidics #HealthcareInnovation #Diagnostics #MarketGrowth #LifeSciences #MedicalDevices #PointOfCare #DrugDelivery #MicrofluidicComponents
'Establishment and evaluation of a naked-eye diagnostic assay for tuberculosis utilizing reverse isothermal amplification-assisted CRISPR-Cas in resource-limited settings' - an AboutScience publication on #ScienceOpen:
🔎 https://www.scienceopen.com/document?vid=b5e93691-e4f6-4332-9e09-12feaf2154b2
#Tuberculosis #MolecularDiagnostics #GlobalHealth #PointOfCare
<div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st1"> <!-- named anchor --> </a> <h5 class="section-title" id="d4671853e161">Introduction:</h5> <p dir="auto" id="d4671853e163">The current scenario of tuberculosis (TB) caused by <i>Mycobacterium tuberculosis</i> (MTB) has presented an almost insurmountable challenge to hospitals with high patient numbers. Delayed diagnosis of TB is a major hurdle in preventing the employment of efficient therapeutics, leading to the development of drug resistance. Hence, an easily accessible diagnostic method, particularly for resource for resource-limited settings, is pertinent for the rapid identification of MTB-infected patients. In pursuit of developing such an assay, the present study offers a CLAP-TB (CRISPR-Cas coupled RT-LAMP Amplification Protocol for Tuberculosis) assay, which will allow us to diagnose TB rapidly and visually. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st2"> <!-- named anchor --> </a> <h5 class="section-title" id="d4671853e169">Methods and results:</h5> <p dir="auto" id="d4671853e171">Herein, the visual MTB detection consists of a method utilizing 232 different samples (sputum, urine, serum) from 82 patients for reverse transcription loop-mediated isothermal amplification (RT-LAMP). Additionally, the assay also utilizes the integration of a CRISPR-Cas12-based system using different guide RNAs of <i>IS6110</i> and an internal control <i>POP7</i> (human RNase P) genes along with visual detection via lateral flow readout-based dipsticks with the unaided eye (~134 min). Overall, the limit of detection for CLAP-TB assay was up to 1 ag of RNA, while the clinical sensitivity and specificity were 98.27% and 100%, respectively, on the pilot scale. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st3"> <!-- named anchor --> </a> <h5 class="section-title" id="d4671853e180">Conclusion:</h5> <p dir="auto" id="d4671853e182">Together, our CLAP-TB assay offers proof of concept for a rapid, sensitive, and specific method with the minimum technical expertise required for TB diagnosis in developing and resource-limited settings. </p> </div>
Ob Keime, Bakterien oder andere Krankheitserreger - die Zahl der Lebensmittelrückrufe in Supermärkten ist jedes Jahr nicht unerheblich.
Durch die Anwendung der POCT-PCR für industrielle Anwendungen zielen wir auf die frühzeitige Erkennung von Krankheitserregern in Wasser sowie auf die Extraktion und Konzentration von Mikroorganismen in Lebensmitteln und Wasser.
Mehr Informationen in unserem Jahresbericht 2022/23 Seite 19 👉 https://www.imm.fraunhofer.de/content/dam/imm/de/documents/PDFs-neu2018/Allgemein/Annual-Report-2022-2023.pdf