Mihail ZdravkovFeb 11

Does anyone know if an NVidia RTX 4060 can handle an Mk1D MinION with runtime basecalling? ONT's documentation says that the minimum requirement is an RTX 5060, but this sounds a bit crazy. And the difference between the two GPUs is not that big I think.

Also, this will mostly be for dRNA-seq, where we basecall after the run, so would it be okay to run two MinIONs in parallel (without basecalling)?

#nanopore #nanoporeSequencing #OxfordNanopore

UnaMar 12, 2025
#nanopore #OxfordNanopore #NanoporeSequencing so just noticed that the 48 pack flowcells is no longer available from the Nanopore store. That is a big price hike for sequencing facilities who usually orders a big pack at a time. @nanopore
Dave LuntAug 5, 2024
We’ve now got a new lab superstition: running the #OxfordNanopore on the whale tiles will lead to much longer reads, because of course it will
rzolauMay 20, 2024
Happy to share a new release of SMARTEn (System for Mobile Analysis in Real-Time of ENvironment) and our high performance metagenomic classifier Coriolis. Please visit https://cse.buffalo.edu/~jzola/smarten/ https://gitlab.com/SCoRe-Group/smarten https://gitlab.com/SCoRe-Group/smarten/-/tree/master/coriolis and have fun! #metagenomics #oxfordnanopore #bioinformatics
SMARTEn

GenomiqueENSMar 7, 2024

#Nanopore

📣 Que quelques places ! 4 sessions de formation destinées aux personnes disposant ou souhaitant acquérir un séquenceur
#OxfordNanopore #MinION ou #P2solo : 📷 4 avril, 27 mai (expériences) 📷 5 avril, 28 mai (bioinfo). Conseil : n'attendez pas😉

👉 https://genomique.biologie.ens.fr/fr/actualites_et_publications/emplois_et_formations

GenomiqueENS – Formations

GenomiqueENS est la plateforme génomique de l’Institut de Biologie de l’École normale supérieure (IBENS).

Zannah SalterSep 25, 2023

In case any other MinION users haven't heard yet (it was news to me this morning!)

You should protect your flowcell from ambient light once the library's loaded. Ten minutes of light exposure impairs the yield, especially R10.4.1 (see link below for details)

Light shields are to be shipped with flowcells in future, but you can probably figure out a way to run your MinION in the dark in the meantime if necessary 😊

https://help.nanoporetech.com/en/articles/8304478-why-do-i-need-to-put-a-light-shield-on-my-flow-cell

#OxfordNanopore #minion #sequencing

Why do I need to put a light shield on my flow cell? | Oxford Nanopore Technologies Help Center

Show threadDr. Eingekochtes WasserJul 21, 2023

Ahja vielleicht noch eine interessante Info:

Die Daten zu den verschiedenen SarsCoV2 Varianten, stammen von quasi jeder aktuell existenten Methode. Sowohl long und short reads als auch gepolishte Daten. Mithilfe der #bioinf konnten all diese Daten kombiniert werden, obwohl die Techniken teils sehr unterschiedlich sind.

Wer sich eingehender zu den aktuell genutzten Techniken informieren will, ist bei #illumina, #pacbio und #oxfordnanopore sequencing gut aufgehoben.

michaelMay 9, 2023
#Oxford Cafe Scientifique today is about #nanopore sequencing of #DNA, #RNA http://www.rowingservice.com/cafescientifique/oxford/ #science #outreach #events #OxfordNanopore
Cafe Scientifique Oxford

Victor A. AlbertJan 1, 2023
First #OxfordNanopore draft #genome for the incredible #Phragmipedium kovachii complete :) Assembled by @michaelarichter
Victor A. AlbertDec 15, 2022

Our preprint on #OxfordNanopore #genome sequencing, assembly, and analysis of three critically endangered ash (Fraxinus) species in the olive family (Oleaceae) is up on #ResearchSquare :)

https://www.researchsquare.com/article/rs-2350866/v1

High quality long-read genomes produced from single MinION flow cells clarify polyploid and demographic histories of critically endangered ash species (Fraxinus: Oleaceae)

With populations of threatened and endangered plants and animals declining worldwide, it is important that high quality genomic records of these species are preserved before they are lost forever. Here, we demonstrate that data from single Oxford Nanopore Technologies (ONT) MinION flow cells c...