ScientiaOct 14
USP participa de frente parlamentar que amplia combate à tuberculose em Ribeirão Preto
- cijnusp
https://jornal.usp.br/campus-ribeirao-preto/usp-participa-de-frente-parlamentar-que-amplia-combate-a-tuberculose-em-ribeirao-preto/
#CampusRibeiroPreto #FrenteParlamentardeCombateTuberculose #Mycobacteriumtuberculosis #Tubercoluse
USP participa de frente parlamentar que amplia combate à tuberculose em Ribeirão Preto

Universidade integra iniciativa que une poder público e Rede-TB para fortalecer políticas e ampliar o diagnóstico precoce

Jornal da USP
StructBiolCommunicationsMay 8, 2025
Structural analysis of M. tuberculosis Rv2173 reveals a canonical class I diterpene synthase fold and supports its annotation as a short-chain product-length synthase #MycobacteriumTuberculosis #IsoprenylDiphosphateSynthase #Rv2173 https://doi.org/10.1107/S2053230X25002298
Structures of Mycobacterium tuberculosis isoprenyl diphosphate synthase Rv2173 in substrate-bound forms

We report the structure of M. tuberculosis isoprenyl diphosphate synthase Rv2173 in three forms, including two with substrate (isoprenyl diphosphate and dimethylallyl diphosphate) occupying the allylic substrate site in different binding poses, with different numbers of metal ions bound. The homodimeric structures possess a canonical all-α-helical trans-isoprenyl diphosphate synthase fold, which supports small but significant differences, notably in the ordering of the C-terminus that closes the active site.

Acta Crystallographica Section F
StructBiolCommunicationsApr 10, 2025
S-Adenosyl-N-decyl-aminoethyl is identified as a promising potential inhibitor for the SAM-dependent mycolic acid synthase UmaA from Mycobacterium tuberculosis #SAMDependentMycolicAcidSynthase #UmaA #MycobacteriumTuberculosis https://doi.org/10.1107/S2053230X25001530
Crystal structure of the S-adenosylmethionine-dependent mycolic acid synthase UmaA from Mycobacterium tuberculosis

The crystal structure of UmaA from M. tuberculosis was solved to 1.95 Å resolution in space group P3221.

Acta Crystallographica Section F
Scientific EuropeanDec 21, 2024

Levofloxacin for preventive treatment of Multidrug Resistant Tuberculosis (MDR TB)
Multidrug resistant tuberculosis (MDR TB) affects half a million people each year. ...........
#ClinicalTrial #Levofloxacin #MDR #MDRTB #MultidrugresistantTB #Multidrugresistanttuberculosis #Mycobacteriumtuberculosis #TB #TBCHAMPTrial #Tuberculosis #VQUINtrial
Umesh Prasad

https://www.scientificeuropean.co.uk/medicine/levofloxacin-for-preventive-treatment-of-multidrug-resistant-tuberculosis-mdr-tb/

Levofloxacin for preventive treatment of Multidrug Resistant Tuberculosis (MDR TB)

Multidrug resistant tuberculosis (MDR TB) affects half a million people each year.  Levofloxacin is advised for preventive treatment based on observational dat

Scientific European
ETIDIoHNov 5, 2024

Source: EBio Medicine, https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(24)00460-2/fulltext

Summary
Background
To date, global priorities for new vaccine R&D have not been systematically identified for endemic pathogens. As part of Immunisation Agenda 2030 (IA2030), we have systematically identified priority endemic pathogens for new vaccine R&D based on country and regional stakeholder values to address this need.

Methods
MCDA surveys targeting policy makers and immunisation stakeholders in each World Health Organization (WHO) region were used to weight eight criteria for prioritisation. Applying those weights to regional pathogen data yielded regional top ten pathogen lists, which are intended to inform regional deliberations on R&D priorities. The regional top ten lists were combined into an IA2030 global priority list. To inform R&D, use cases for new vaccines and monoclonal antibodies were identified, then categorized in terms of the activities needed to accelerate progress.

Findings
In five out of six WHO regions, Annual deaths in children under five and Contribution to antimicrobial resistance were the most heavily weighted criteria. How participants weighted the criteria was not associated with their region, biographical characteristics, or areas of expertise. Five pathogens were common priorities across all regions: M tuberculosis, HIV-1, K pneumoniae, S aureus, and Extra-intestinal pathogenic E coli. Six pathogens were priorities in single regions. Combining regional top ten lists provided a global list of 17 priority pathogens for new vaccine R&D. Thirty-four distinct use cases were identified for new products targeting these pathogens. While most are in the “Advance product development” category, ten are in the “Research” category and seven are in the “Prepare to implement” category.

Interpretation
These priorities for new vaccine R&D will help stakeholders better respond to regional and country needs. The use cases will inform R&D and enable monitoring of R&D under IA2030.

____

https://etidioh.wordpress.com/2024/11/05/identifying-who-global-priority-endemic-pathogens-for-vaccine-research-and-development-rd-using-multi-criteria-decision-analysis-mcda-an-objective-of-the-immunization-agenda-2030/

#abstract #bacteria #eColi #health #HIVAIDS #infectiousDiseases #klebsiellaPneumoniae #mycobacteriumTuberculosis #news #research #science #staphylococcusAureus #vaccines #WHO

StructBiolCommunicationsJul 8, 2024
The N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv was crystallized and its ligand-binding abilities were analyzed @ActaCrystF @IUCr #MycobacteriumTuberculosis #Rv1421 #WalkerABLikeMotif
https://t.co/1OJYXJjbyW
Preliminary X-ray diffraction and ligand-binding analyses of the N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv

A crystal of the N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv (MtRv1421-NTD) diffracted to 1.7 Å resolution. MtRv1421-NTD, which contains a Walker A/B-like motif, can bind uridine diphosphate (UDP) and UDP-N-acetylglucosamine, indicating that the presence of a UDP moiety on the ligand may be essential for its interaction.

Acta Crystallographica Section F
Peer Community JournalSep 29, 2023
Now published in Peer Community Journal, #evolutionarybiology section: How do #monomorphicbacteria evolve? The #Mycobacteriumtuberculosis complex and the awkward #populationgenetics of extreme clonality https://doi.org/10.24072/pcjournal.322
How do monomorphic bacteria evolve? The Mycobacterium tuberculosis complex and the awkward population genetics of extreme clonality

StructBiolCommunicationsAug 15, 2023
The structure of FadD23 from M. tuberculosis complexed with the general inhibitor PhU-AMS sheds light on the design of inhibitors of FadD23 and of fatty acyl-AMP ligases @IUCr #MycobacteriumTuberculosis #Sulfolipid1Synthesis #FattyAcylAMPLigase https://doi.org/10.1107/S2053230X23005836
Structural basis for the development of potential inhibitors targeting FadD23 from Mycobacterium tuberculosis

The crystal structure of M. tuberculosis FadD23 complexed with the inhibitor 5′-O-[N-(11-phenoxyundecanoyl)sulfamoyl]adenosine was solved at 2.64 Å resolution and compared with similar three-dimensional structures. It is proposed that the inhibitor blocks substrate transfer from FadD23 to polyketide synthase 2.

Acta Crystallographica Section F
Peer Community InJun 30, 2023
A new #preprint #OpenScience #PeerReview by #PCIEvolBiol: Christoph Stritt, Sebastien Gagneux (2023) How do #monomorphicbacteria evolve? The #Mycobacteriumtuberculosis complex and the awkward #populationgenetics of extreme clonality. #EcoEvoRxiv https://doi.org/10.32942/X2GW2P 🔽
How do monomorphic bacteria evolve? The Mycobacterium tuberculosis complex and the awkward population genetics of extreme clonality

eLifeMar 22, 2023
The method used to prepare #MycobacteriumTuberculosis cells affects the bacterial cell wall, changing infection outcomes. #Tuberculosis https://elifesciences.org/articles/85416?utm_source=mastodon&utm_medium=social&utm_campaign=organic
Single cell preparations of Mycobacterium tuberculosis damage the mycobacterial envelope and disrupt macrophage interactions

The experimental methods that are routinely used to disperse mycobacterial aggregates markedly impact macrophage infection outcomes, which should be taken into account to appropriately interpret host-pathogen interactions studies.

eLife