Mastodawn

Comparative Analysis of the Complete Mitochondrial Genome of Apis laboriosa (Insecta: Hymenoptera: Apidae) - #Apislaboriosa #completemitogenome #phylogeneticrelationship #Apis #mitogenome #Apidae #mitochondria #mitochondrialgenome - https://link.springer.com/article/10.3103/S0095452725060076

Comparative Analysis of the Complete Mitochondrial Genome of Apis laboriosa (Insecta: Hymenoptera: Apidae) - Cytology and Genetics

Abstract Apis laboriosa Smith is the largest bee in the genus Apis, and it is mainly distributed in Yunnan and Tibet regions in China. In this study, two complete mitogenomes of A. laboriosa from Luding and Dehong were obtained through Illumina sequencing, and the phylogenetic relationship among different geographical populations of A. laboriosa was determined. The entire mitogenomes of A. laboriosa are 15 546 (Luding) and 15 239 (Dehong) bp in length, respectively. The A. laboriosa mitogenome contains 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a control region rich in A + T. The maximum likelihood phylogenetic tree using 13 PCGs reveals that A. laboriosa from Dehong and Shangri-La clustered together and form a sister taxon to Luding. This study provides data for an in-depth exploration of the genetic diversity of A. laboriosa and layes a scientific foundation for the conservation of this species.

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Cytology and Genetics Aug 14, 2025

The Complete Mitochondrial Genomes of Two Apple Cultivars Reveal the Highly Conserved Structure in Cultivated Apples - #cultivatedapple #mitochondrialgenome #mitochondrialplastidsequence #RNAeditingsites #phylogeneticanalysis #apple #mitochondria #sequence #RNAediting #CompleteMitochondrialGenomes - https://link.springer.com/article/10.3103/S0095452725020057

The Complete Mitochondrial Genomes of Two Apple Cultivars Reveal the Highly Conserved Structure in Cultivated Apples - Cytology and Genetics

Abstract Apples are one of the most popular and economically important fruits worldwide and have both nutritional and ornamental value. However, because of their complexity, studies on apple mitochondrial (mt) genomes have been limited. In this study, the mt genomes of Aksu Fuji and Ralls Janet cultivars were assembled, annotated, and analyzed based on a hybrid strategy using Illumina, and comprehensive comparisons of their structure, gene content, intercellular gene transfer, phylogeny, and RNA editing sites were performed. The mt genome length and gene structure of the two apple cultivars were identical (396 592 bp) and included 63 protein-coding genes (PCGs), 20 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes. There were 44 and 31 mitochondrial plastid fragments (MTPTs) identified between the mt and plastid genomes of the Ralls Janet and Aksu Fuji cultivars, accounting for 1.98 and 2.19% of their mt genomes, respectively. Furthermore, there were 419 and 421 RNA editing sites were detected in Aksu Fuji and Ralls Janet, respectively. Analyses of coding usage bias, nucleotide diversity, selection pressure, and genetic distance revealed that the mt genomes of the two cultivars were highly conserved. Phylogenetic analysis of 29 Rosaceae species showed that Aksu Fuji and Ralls Janet clustered with Malus baccata and M. kansuensis. This study provides new insights into the genetics, systematics, and evolution of apple mt genomes.

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PLOS Biology Jun 25, 2025

What is the therapeutic potential of correcting mutations by base editing of the #MitochondrialGenome? This study shows that #mitochondrial #BaseEditing can indeed functionally create & correct mitochondrial pathogenic #mutations in patient-derived cells @PLOSBiology https://plos.io/3ZMa2K2

Cytology and Genetics Jun 17, 2025

Complete Mitochondrial Genome of the Ossuaria sichuanensis (Hemiptera: Cicadellidae: Typhlocybinae) from China and Its Phylogenetic Implications - #leafhopper #Ossuariasichuanensis #completemitochondrialgenome #phylogeneticanalysis #China #Ossuaria #mitochondrialgenome #genome - https://link.springer.com/article/10.3103/S0095452725010165

Complete Mitochondrial Genome of the Ossuaria sichuanensis (Hemiptera: Cicadellidae: Typhlocybinae) from China and Its Phylogenetic Implications - Cytology and Genetics

Abstract Leafhoppers, a broad group of Hemiptera insects, are economic pests that wreak havoc on agriculture and forestry. The genus Ossuaria was established by Dworakowska in 1979, Zhang and Yang discovered Ossuaria sichuanensis (Zhang and Yang, 2011) from China in 2011, but there is no molecular biological evidence to record the genus. To report complete mitochondrial genome of O. sichuanensis, DNA was extracted from the muscle tissue of adult insect and sequenced. The original data were assembled and annotated, a complete mitochondrial genome was mapped, and phylogenetic analysis was performed using maximum likelihood and Bayesian inference. The circular mitogenome of O. sichuanensis is 15.558 bp in length and including 37 typical genes (13 PCGs, 22 tRNA genes, and 2 rRNA genes) and a putative control region. The overall base composition is 42.89% A, 36.92% T, 11.02% C, and 9.17% G, and the A + T content is 79.81%. Phylogenetic analyses confirmed the O. sichuanensis is the sisters of other members of the tribe Erythroneurini. The mitochondrial genome structure agrees with earlier research. The genus Ossuaria has been characterized at the molecular level for the first time, and additional research is required to determine how the tribe Zyginellini and the tribe Typhlocybini are related.

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