Fatih DemirCombining all of this data into a fabulous circle plot clearly pointed towards an important role of the cleavage at pos. 1514, generating C3-LHF1. This became the focus of our future efforts. #LHF1 #CirclePlot 6/n
Looking at proteolytis in human plasma, we observed a lot of cleavages in complement C3, with known cleavages for C3a, C3b, and C3(d)g - but also a yet not deeply investigated cleavage at pos. 1514, which represented for us a quite intriguing lupus-enriched cleavage, thus we named it C3-LHF1 for complement "C3 Lupus Human Fragment 1" #C3 #Complement #LHF1 #SLE #Lupus 3/n
After a looong journey, I can present you the results of a deep & thorough investigation of human plasma proteolysis by mass spec: SLE N-terminomics paper with a complement C3 fragment(s) is out now in EMBOJ: https://www.embopress.org/doi/full/10.1038/s44318-025-00598-8 - highlights: a nice resource on proteolytically processed N-termini in human plasma of healthy & SLE patients, with a lot of complement bonus for all interested scientists. #SLE #Science #Proteomics #TeamMassSpec #InflammatoryDisease #LupusResearch #C3 #LHF1 1/10
