PLOS BiologyMay 8, 2024
#Toxoplasma relies on specialized secretory organelles for invasion & host cell manipulation. @ToxInLA &co identifies the TBC domain protein TBC9 as an essential regulator of the #GTPase Rab2 that controls intracellular vesicular trafficking #PLOSBiology https://plos.io/3Upgkvu
Systematic characterization of all Toxoplasma gondii TBC domain-containing proteins identifies an essential regulator of Rab2 in the secretory pathway

Toxoplasma gondii regulates vesicle trafficking by secreting Rab GTPases from specialised organelles. This study characterizes all Tre2-Bub2-Cdc16 (TBC) domain-containing proteins in T. gondii, identifying TBC9 as an essential regulator of Rab2 that controls intracellular vesicular trafficking.

MPI for Marine MicrobiologyFeb 28, 2024

#Microbes like comics, too!💬
#RNA is the language, #extracellularvesicles serve as speech bubbles.
Out now
@PNASNews by Joshua Mills, Susanne erdmann et al.

Read more: https://mpi-bremen.de/en/Microbial-comics-RNA-as-a-common-language-presented-in-extracellular-speech-bubbles.html

Full paper: https://pnas.org/doi/10.1073/pnas.2311321121

#archaea #GTPase #EV #evolution

Microbial comics: RNA as a common language, presented in extracellular speech-bubbles

The EMBO JournalJan 25, 2024
Structural basis of oligomerization of antimicrobial #dynamin-related GBP1 #GTPase: a nucleotide-dependent conformational change facilitates formation of protein coats around pathogenic bacteria
Oliver Daumke and coworkers
https://www.embopress.org/doi/full/10.1038/s44318-023-00023-y
eLifeOct 1, 2023
August's most-read Tools paper presents an #optogenetic toolbox to reversibly control Rho #GTPase activity for manipulating the barrier between #endothelial cells: https://elifesciences.org/articles/84364?utm_source=mastodon&utm_medium=social&utm_campaign=submissions_organic
Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.

eLife
The EMBO JournalAug 30, 2023

TOR-mediated Ypt1 #phosphorylation regulates autophagy initiation complex assembly

The Rab #GTPase #Ypt1 serves as a bridge that connects environmental sensing to the formation of the #autophagy initiation complex.

https://www.embopress.org/doi/full/10.15252/embj.2022112814

Journal of Experimental BotanyAug 25, 2023

🌽 #Kernels are an important focus for improving #yield and quality
🌽 Defective kernel 66 encodes a #GTPase essential for kernel #development in #maize
🌽 Here characterises the candidate gene #DEK66...

📖 https://doi.org/10.1093/jxb/erad289

#Endosperm #mitochondria #ribosome #mutant

Defective kernel 66 encodes a GTPase essential for kernel development in maize

DEK66 encodes a mitochondrial ribosomal assembly factor and is involved in the regulation of maize kernel development by affecting the morphology and function o

OUP Academic
The EMBO JournalAug 23, 2023
The #IFT81-#IFT74 complex acts as an unconventional #RabL2 #GTPase-activating protein during #intraflagellar transport
Esben Lorentzen and coworkers
https://www.embopress.org/doi/full/10.15252/embj.2022111807
The EMBO JournalAug 17, 2023
#Ovariancancer cells lacking tumor suppressor #PTEN require an ARF #GTPase module for #integrin-dependent #invasiveness
A #RefereedPreprint c/o @ReviewCommons by Konstantina Nikolatou, David Bryant et al
https://www.embopress.org/doi/full/10.15252/embj.2023113987
The EMBO JournalJan 16, 2023

Cover feature in issue 42/2:

#Golgi #oxygen sensor controlling #intestinal ‘mucin #glycosylation
by Tal Ilani, Deborah Fass and colleagues with SciStories.com

And:
Gut colonization via #GTPase-less EF-G paralog
#Yeast cells in two minds about #cellcycle start
#MASTL/#PP2A module restraining #PI3K-Akt
Zinc-dependent structural transition in ALS-mutated #SOD1
https://www.embopress.org/toc/14602075/2023/42/2

Show threadJoseph P.Dec 19, 2022
This creates layers of poultry, which is similar to the double #membrane /double space structure of #mitochondria. The author then explains that the process of mitochondrial fusion involves connecting the two mitochondria layer by layer, using proteins called mitofusins. Mitofusins have a #GTPase domain, two hydrophobic heptad repeat coiled-coil domains, and a small hydrophobic transmembrane domain.