Voilà, c'est décidé, je met la famille #hexaconique en sommeil, J'ai l'impression de tourner en rond, alors j'arrete le dèv, et je vais passer tout ça dans #konrad pour raytracer le #flim en 1920x1080. ETA : dans deux mois.

http://maison.tth.netlib.re/v/hc/the-hexacone-family.mp4

https://git.tetalab.org/tTh/HexaCone/commit/f3df71d687b2bf77d6383ab9f6928a714cf14fe4

To capture this local heterogeneity, the authors developed a #multiphoton #FLIM approach with the Na⁺ indicator ING-2 and #astrocyte labeling, enabling quantitative Na⁺ measurements in individual #astrocytes and their processes. This is important because astrocytic Na⁺ is not just a bulk somatic variable. It varies locally across branches and subdomains, where K⁺ uptake and #glutamate-linked Na⁺ influx are regulated.

#Neuroscience #Imaging

🧠🎨 New paper by Meyer et al: #astrocytic #sodium #homeostasis is not uniform. Using multiphoton #FLIM in #mouse #brain slices and #invivo, they show strong #cellular and #subcellular heterogeneity in astrocytic Na⁺ levels.

Processes contain more Na⁺ than somata, Na⁺ varies between #astrocyte branches, and distinct Na⁺/K⁺-ATPase subunit patterns help tune local K⁺ uptake and #glutamate-linked Na⁺ influx.

🌍 https://doi.org/10.1038/s41467-026-73435-z

#Neuroscience #Astrocytes #Neurobiology #NeuralDynamics

#Methode
Fluoreszenz-Marathonläufer vs. Sprinter: Forschende der @unigoettingen
nutzen #FluoreszenzlebensdauerMikroskopie & HaloTag-Nanobodies, um Zielproteine zu detektieren und trotz spektraler Überlappung exakt auseinander zu halten.

Mehr im Artikel von Andrea Pitzschke: https://www.laborjournal.de/editorials/3450.php

#Laborjournal #LifeSci #FLIM #Nanobodies #HaloTag #Mikroskopie #Zellbiologie #MultiplexImaging #Immunofluorescence

Connaissez-vous

"Colossus - The Forbin Project" (1970) ?

Ce chef-d'œuvre de science-fiction (un peu niche) a apparemment disparu des plateformes de streaming. Certains pensent que ce serait du fait de Melon Husk: https://www.horroranthologymovies.com/science-fiction-podcast/did-elon-musk-remove-ai-sci-fi-film-colossus-the-forbin-project/

Dans tous les cas, il ne faut pas tolérer la censure d'un #flim génial dont le thème central est plus pertinent que jamais!

(re-)matons-le, et partageons-le! #bittorent https://thepiratebay.org/description.php?id=78958142 et https://archive.org/details/colossus-the-forbin-project-1970_202508

#cinema #cinemastodon #scifi

MAETTA . TRANSMISSION @ TBA - 07 Feb feat. Jayzo, FLIM

#SESH #Jayzo #FLIM

https://sesh.sx/e/1613130

Una investigación en IMDEA Nanociencia, CBM & CNB (CSIC) revela cómo las bacterias "sienten" superficies.

🧫🤏Usando microscopía de fluorescencia #FLIM las científicas observan los cambios en la tensión de la membrana en tiempo real.

https://nanociencia.imdea.org/es/imdea-nanociencia/noticias/item/an-advanced-fluorescence-microscopy-technique-reveals-how-bacteria-feel-surfaces
#IMDEAnanoPublications

New research from IMDEA Nanociencia, CNB & CBM reveals how bacteria "feel" the surfaces they land on!

🧫🤏Using fluorescence lifetime imaging microscopy #FLIM, scientists can watch membrane tension change in real time.

+info: https://nanociencia.imdea.org/home-en/news/item/an-advanced-fluorescence-microscopy-technique-reveals-how-bacteria-feel-surfaces
#IMDEAnanoPublications

Haven't been posting here in a while; just too busy! Hoping to get back to it. Here's the latest of what I (and my creative, cooperative collaborators have been up to:

https://metapsychosis.com/?na=v&nk=2-cda7977fb0&id=105

#literature #writing #sf #scifi #trans #cinema #flim #hitchcock #vertigo #acousmatic #music #philosophy #community