Expansion microscopy with sub-molecular resolution:

"we present 1000ExM, a four-network IPN (interpenetrating polymer network) formed by an initial ionic gel and three successive ionic-in-ionic casting steps. This process yields ~1000-fold expansion in each dimension while preserving three-dimensional (3D) molecular geometry."

"Physical expansion converts sub-nanometer-scale molecular distances into micrometer-scale separations that are resolvable using standard light microscopy, enabling direct readout of 3D amino acid positions without the need for averaging."

"Thousandfold Expansion Microscopy", Hu et al. 2026 (Boyden lab)
https://www.biorxiv.org/content/10.64898/2026.05.31.729018v1

#ExM #microscopy

"Charting the landscape of cytoskeletal diversity in microbial eukaryotes", Mikus et al. 2025 (Dudin lab)
https://www.cell.com/cell/fulltext/S0092-8674(25)01127-4

CC @davi

#CellBiology #cytoskeleton #ExM

Solar en Poloid

https://musicmustbe.nl/blog/2025/03/07/solar-en-poloid/

#NieuweMuziek #ambient #bandcamp #electronica #exm #IDM #TouchedMusic

Today I feel like a plant, static but alive

#ArabidopsisRoot #microscopy #ExM

Good morning!! Cell biology rocks, isn't it?

#microscopy #exm #cellbiology

There some of my specialities, enjoy!

#imaging #microscopy #neuroscience #superesolution #expansion #exm

#ExIGS: Combining #expansion #microscopy (#ExM) & in situ #genome #sequencing (#IGS) enables sequencing of #genomic #DNA & #superresolution localization of #nuclear #proteins in single #cells:

Preprint: https://doi.org/10.1101/2024.09.24.614614
#DIYbio #lab #instruments #NGS #fluorescence #imaging