Resuscitation of Hamsters after Supercooling or Partial Crystallization at Body Temperatures Below 0° C. - Nature

A VARIETY of cells and tissues of mammals survive for long periods at the temperature of ‘dry ice’ − 79° C., when frozen in media containing glycerol1. At the other extreme, Andjus's work on the whole animal shows that, by special methods of cooling and rewarming, rats can be revived from deep body temperatures of about + 0.5° C.2. We are now attempting to close the gap between these two lines of work (a) by increasing the size and complexity of the isolated tissue cooled to − 79° C., as, for example, by freezing a whole isolated organ3, and (b) by reducing the whole animal to a body temperature below zero. This communication deals with experiments on the second of these developments.

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Successful use of high-pressure freezing for cell cryopreservation

A high-pressure method of instantaneously freezing cells proves to be effective in the first empirical validation of its kind.

Frozen Ark - Wikipedia

Frozen zoo - Wikipedia

The protein that helps plants survive the cold

As climate conditions become increasingly unpredictable, understanding how plants respond to cold is crucial for securing future crop resilience. In her doctoral thesis, Varvara Dikaya at Umeå University has studied the protein PORCUPINE, which plays a central role in how plants adapt to low temperatures. Her research reveals that this protein is part of multiple intertwined signaling pathways that help plants survive.

Frozen Ark - Wikipedia

Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation - Scientific Reports

This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage. The findings reveal that exposure to low CPA concentrations (0.5 M) did not induce significant damage to eggs. However, high concentrations (3 M) proved highly detrimental. Every cryopreservation approach investigated in this study resulted in irreversible damage to the sea urchin eggs, rendering them nonviable for future use. The research sheds light on the importance of understanding the structural alterations induced by CPAs and cryopreservation methods. This knowledge is essential for refining cryopreservation methods, potentially paving the way for successful preservation of these challenging cells.