Bungo AkiyoshiKubalová and collleagues used PALM/SIM to count the number of CENH3 in barley centromeres, estimating ~13,500 molecules per centromere #Chromosoma
Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes - PubMed
Topoisomerase IIα (Topo IIα) and the centromere-specific histone H3 variant CENH3 are key proteins involved in chromatin condensation and centromere determination, respectively. Consequently, they are required for proper chromosome segregation during cell divisions. We combined two super-resolution …