CellBioNewsJan 27, 2023

An open-source stopwatch to time interactions between #molecules inside living #cells.

#Cytology #cytochemistry #BrightEyes_TTM

https://phys.org/news/2023-01-open-source-stopwatch-interactions-molecules-cells.html

An open-source stopwatch to time interactions between molecules inside living cells

A stopwatch to investigate what happens inside living cells at a thousandth of a millisecond scale: this is the open-source platform BrightEyes-TTM developed by the research team led by Giuseppe Vicidomini at Istituto Italiano di Tecnologia (IIT-Italian Institute of Technology).

Phys.org
VicidominiLabDec 12, 2022
A great work deserves a great cakes.....maybe two!
Thanks @EliSlenders@twitter.com for bringing some Belgium receipts in Italy.
#BrightEyes_TTM #singlephotonmicroscopy #BrightEyes_ERC #SPAD #SPAD_FFS #imagescanningmicroscopy #fluorescence #lifetime #quantum
https://doi.org/10.1038/s41467-022-35064-0
Show threadVicidominiLabDec 7, 2022
Fluorescence has never been so informative! Thanks to the synergy between the #BrightEyes_TTM and the #SPAD array detector, we can perform #FLFS on living cells to simultaneously follow different dynamics parameters and fluorescence lifetime. (4/5)
#singlephotonmicroscopy
Show threadVicidominiLabDec 7, 2022
We characterized the #BrightEyes_TTM. We can reach a single-shot precision of 30 ps, 4 ns dead-time, and an "infinite" range in all channels, with a total flux of up to 125 MHz. (3/5)
#TCSPC #antibunching #quantum #microscopy #FPGA
#coding #LIDAR
VicidominiLabDec 7, 2022
#quantum is here! Why not collect #fluorescence #photon-by-photon in your laser-scanning #microscope? We developed the #BrightEyes_TTM, an #opensource data acquisition system able to do it!
https://www.nature.com/articles/s41467-022-35064-0
@IITalk@twitter.com @ERC_Research@twitter.com @RNA_iit@twitter.com @MSCActions@twitter.com #BrightEyes_ERC
(1/5)
The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy - Nature Communications

The authors developed an open-source, low-cost, multi-channel time-tagging module for fluorescence lifetime image scanning microscopy and correlation spectroscopy that can tag in parallel multiple single-photon events with 30 ps precision.

Nature