Poly-L-Lysine (PLL) mediates the non-specific adhesion of cells and is commonly used in Atomic Force Microscopy (AFM) measurements, to ensure that cells remain attached to the substrate. However, it is acknowledged that adhesion affects the measured mechanical properties, in particular in the case Red Blood Cells (RBCs). This results in a wide range of Young's modulus E reported in the literature. The present study aims at providing a systematic approach to the impact of non-specific adhesion on the rheology of RBCs. It provides a correlation between the topography profile of adherent RBCs and their rheology, from weak (cPLL=10-3 mg/mL) to strong-adhesion (cPLL=10 mg/mL) regimes. Using RICM and AFM, we find that there is a continuum of RBC shapes promoted by adhesion, from concave to dome-shaped, as predicted by the theory of vesicle adhesion. Their elastic properties discriminate them into two populations depending on adhesion strength, where stiffer RBCs (Eā100 Pa) correlate with dome-shaped cells. These findings are supported by rheology measurements of the dynamic complex shear modulus G*(f): while the storage modulus increases with cell-substrate adhesion, reflective of an increased membrane shear modulus, the loss modulus remains unchanged. Finally, further analysis inspired by membrane theory shows that different deformation modes may be triggered during indentation of either weakly or strongly adhering RBCs, illustrating the limits of the Hertz model. ### Competing Interest Statement The authors have declared no competing interest. Agence Nationale de la Recherche, https://ror.org/00rbzpz17, ANR-21-CE09-0011
The height of viral particles adsorbed on solid substrates is governed by the equilibrium between adhesion energy and capsid elasticity. While the resulting height distribution has been proposed as a non-invasive proxy for viral sti$\hookleftarrow$ness, the physical origin of its broadening is unknown. In this work, we combine Atomic Force Microscopy (AFM) topography measurements of Adeno-Associated Virus (AAV8) and Hepatitis B Virus (HBV) with a theoretical shell-deformation model to identify the determinants of height dispersion. By modeling the viral shell as an elastic body under adhesive load, we evaluate the relative contributions of thermal fluctuations and mechanical heterogeneity to the observed height dispersion. We demonstrate that thermal noise is insu cient to explain the width of the distribution. Instead, the data support a model where the dispersion in height arises from the intrinsic variability of capsid sti$\hookleftarrow$ness. This variability is associated to the surface inhomogeneity of identical capsids. Our results validate that, when this inhomogeneity is accounted for, the height distribution of adsorbed particles provides a quantitative measure of viral mechanics without the need for individual nanoindentation.
Underwater adhesion research increasingly draws on bioinspired systems to uncover the molecular mechanisms that enable strong interfacial binding in aqueous environments. The biofilm adhesin Bap1 from Vibrio cholerae contains a short peptide motif, SYWFFGWHTK (CP), which exhibits exceptional adhesive performance, surpassing mussel foot protein mfp5 under comparable conditions. Despite its promise, the roles of ionic environments and aggregation behavior in governing CP adhesion remain unclear. In this study, we investigate how ion identity influences CP aggregation, film formation, and interfacial properties. Using dynamic light scattering, we identify the formation of micron-scale assemblies of aggregated molecular clusters (AAMCs), with size distributions modulated by salt type. Quartz crystal microbalance with dissipation and liquid atomic force microscopy reveal that CP film formation is both surface- and ion-dependent. On gold substrates, AAMCs preferentially adsorb and collapse into rigid, smooth nanofilms, consistent with hydrophobic-driven compaction. In contrast, silicate surfaces inhibit such collapse, yielding distinct morphologies and interfacial energetics. These findings demonstrate that surface chemistry and ionic conditions jointly regulate peptide aggregation and adhesion. This work provides mechanistic insight into hydrophobic-rich peptide systems and informs the rational design of next-generation wet adhesives, with broader implications for biomaterials and peptide-based formulations. ### Competing Interest Statement J.Y. is named inventor on a related patent through Yale University (US Application Number: 63/376,414). The remaining authors, S.Z., D.R.J.P., N.L.M., A.A., B.H., A.D.M, T.P.C., and R.C.A.E. declare no competing interests. U.S. National Science Foundation, https://ror.org/021nxhr62, NSF-HRD-1547848, NSF-CHE-2316870 National Institutes of Health, https://ror.org/01cwqze88, T32 GM141862 Defense Advanced Research Projects Agency, HR0011-24-3-03-62, HR00112430356 Burroughs Wellcome Fund, 1022835 National Agency for Research and Development of Chile, Fondecyt Regular 1251913
Author summary Living organisms build their bodies through morphogenesis, during which cells autonomously arrange themselves into functional structures such as sheets, tubes, and spheres. From simple monolayered spheres to complex multilayered tissues organized by adhesion, it remains unclear how such diverse forms arise. Here, we mathematically modeled a population of proliferating cells governed only by two microscopic factors: the polarity strength of the cell and the time scale at which polarity is regulated by cell-cell contact. Surprisingly, we found that this minimal model reproduces five basic morphological types observed in living embryos, including monolayer/multilayer structures and two distinct modes of cavity formation: by wrapping around or by inflating from the inside. Systematic simulations revealed that these macroscale outcomes are determined solely by two parameters controlling polarity strength and its regulation, suggesting that simple physical rules underlie diverse developmental architectures. Analysis of the model uncovers phase transitions between the five morphogenetic types and reveals how varying polarity and adhesion can recapitulate features of real embryogenesis. Our work proposes a unified framework that connects microscopic polarity mechanics to diverse developmental morphologies and provides a foundation for future applications in organoid design and tissue engineering.
Rxiv mechanobio