A small yet meaningful gain arrives within your reach. This may come as a refund, favor, or reward for previous effort. Remain alert to subtle signs guiding you toward advantage. Gratitude attracts more abundance before the day ends.
#kipper #fortune #abundance #intuition #dailyguidance #mystic #fate
An opportunity for ease and grace surrounds you today. Approach tasks with confidence and charm, for a small favor or unexpected support may bring tangible benefit. Let gratitude and clear intent guide every choice; your influence is stronger than you think.
Accuracy of occurrence and abundance estimates from insect metabarcoding
#insect #metabarcoding #abundance #preprint
https://www.biorxiv.org/content/10.64898/2026.02.20.707016v1
DNA metabarcoding - high-throughput sequencing of barcode regions from bulk samples - has become a key tool for insect biodiversity assessment. Yet, how methodological choices affect the accuracy of metabarcoding data remains insufficiently explored. In this paper, we ask: (1) How does the lysis method (non-destructive lysis vs. destructive homogenization) affect community recovery? (2) How comprehensively does metabarcoding capture species richness? (3) To what extent can spike-ins improve abundance estimates? (4) How accurately can species abundances be estimated? We evaluated the accuracy of insect metabarcoding using 4,749 bulk samples from a large-scale biodiversity survey subjected to mild lysis. Of these samples, 856 were also homogenized, allowing a systematic comparison of the effect of alternative treatments. To potentially improve abundance estimates, we added six biological spike-ins (i.e., foreign insects) to all samples, and two synthetic spike-ins (artificial DNA fragments) to the homogenization treatment. In addition, we established the contents of 15 samples by individually barcoding all specimens, enabling direct assessment of occurrence and abundance estimates. Our results revealed consistent differences between destructive and non-destructive treatments. While both methods reliably detected the majority of species, small and soft-bodied taxa were more often recovered after mild lysis than after homogenization, while the reverse was true for heavily sclerotized, hairy, and large taxa. Using biological spike-ins for calibration reduced the variance in read numbers per specimen considerably, especially in homogenized samples, while synthetic spike-ins were less effective. In a Bayesian analysis, where species data were matched to the best-fitting spike-in calibration curve, accurate abundance estimates (+/-1 individual) were obtained for 72.9% of species occurrences. Our results show that it is possible to obtain reasonably accurate abundance estimates from metabarcoding data, and that mild lysis and homogenization result in different taxon-specific biases in terms of occurrence data, with neither method outperforming the other. Accuracy is improved by homogenization rather than mild lysis of samples, and by the use of biological rather than synthetic spike-ins. Together, these findings provide a major step towards robust, quantitative biodiversity monitoring using DNA-metabarcoding. ### Competing Interest Statement The authors have declared no competing interest. Knut and Alice Wallenberg Foundation, https://ror.org/004hzzk67, 2017.088 Swedish Research Council, https://ror.org/03zttf063, 2021-04830 National Science Centre, https://ror.org/03ha2q922, 2018/31/B/NZ8/01158, 2021/43/B/NZ8/03376
One day, your blender may make the perfect cocktail, make money for you when it’s idle, and contribute to a global wealth of computing resources.
Read more 👉 https://lttr.ai/Aptaf
【🎉Latest accepted article】
Integrated assessment of invasion risk: coupling habitat #Suitability, population #Abundance and #EcosystemServiceValue for Xanthium spinosum in China
#Prediction | #StructuralEquationModel | #EcosystemServicesValuation
This study presents a novel integrated suitability-abundance-ESV framework, breaking through the limitations of traditional binary species distribution mod
“Habits decide who you become permanently, what you do daily and what you have always.”
― Mensah Oteh
#Bot #Quote #Abundance #Appreciation #Celebration #Dreams #Friendship #Gratitude #Greatness #Growth #Habits #Happiness #Influence #Inspirational #Life #Love #People #Potential #Questions #Relationships #Success #Thinking #Vision
edit: this study circle is now at capacity!
👉 Study Circle: Reading Radical Abundance - How To Win a Green Democratic Future
📅 Starting April 8th, every other Wednesday from 18:30 - 20:30 until June 17th
📍ABF Malmö.
🎟️ Free entry, registration required
Capitalism has created a world of bullshit abundance, where we have too much of what we don’t need and too little of what we do. Through this system’s pursuit of profits, we have been put on a collision course with social and ecological limits that can no longer be ignored. We need an alternative. We need radical abundance. A world of human and non-human flourishing made possible by democratically planned production.
In this study circle we will read Radical Abundance (2025) by Kai Heron, Keir Milburn and Bertie Russell, an eco-socialist book packed with real-life examples of communal planning and resistance, proving a better future is possible. Together we will discuss the examples presented in the book, if they could work in a Swedish context and how they relate to degrowth.
Meeting dates:
8 April, 22 April, 6 May, 20 May, 3 June, 17 June
#studycircle #reading #bookstodon #abundance #degrowth #ecosocialism #skane #malmo
Jeannie Maegenwylf
Teresita Porter 🙋🏻♀️
LBHuston
Journal of Plant Ecology
EarthStarRising
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